HETERODUPLEX ANALYSES
\hˈɛtɹə͡ʊdjˌuːplɛks ɐnˈaləsˌiːz], \hˈɛtɹəʊdjˌuːplɛks ɐnˈaləsˌiːz], \h_ˈɛ_t_ɹ_əʊ_d_j_ˌuː_p_l_ɛ_k_s ɐ_n_ˈa_l_ə_s_ˌiː_z]\
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A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
By DataStellar Co., Ltd
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